Journal: Cellular Oncology (Dordrecht, Netherlands)
Article Title: Upregulated NNMT sustains angiogenesis in clear cell renal cell carcinoma through activation of the SDF-1/CXCR4–ERK–CCL20 axis
doi: 10.1007/s13402-025-01115-z
Figure Lengend Snippet: Increased expression of NNMT is observed in ccRCC and predicts an unfavorable prognosis for patients. ( A ) Heatmap of DEGs in normal samples and RCC samples. ( B ) Heatmap of methylation levels of methylation-driven genes (MDGs). ( C ) Venn diagram revealed that identification of 11 DEMDGs in RCC. ( D ) The expression levels and relative methylation levels of 11DEMDGs. ( E ) Kaplan-Meier survival curve showed the correlation of NNMT expression with survival rate of ccRCC in TCGA database. ( F ) The relative methylation level of NNMT. ( G ) Analysis of NNMT mRNA expression in TCGA-KIRC and GEO ( GSE66272 , GSE105261 ) datasets. ( H ) Scatterplot showing the fold change in NNMT mRNA levels in ccRCC and corresponding paracarcinoma specimens ( n = 20). ( I ) Western blot of NNMT expression in 9 pairs of ccRCC and corresponding paracarcinoma specimens. ( J ) IHC of NNMT expression in ccRCC and corresponding paracarcinoma specimens. The data are presented as a representative image. Scale bar, 50 μm. K . Correlation between overall survival (OS) and the relative expression level of NNMT in patients with ccRCC. L . Western blot of NNMT expression in the HK2 and RCC cell lines. Data were analyzed using Student’s t-test or Mantel-Cox. Significant results were presented as * P < 0.05, ** P < 0.01, *** P < 0.001
Article Snippet: The ccRCC cell lines 769-P, 786-O, OS-RC2, and ACHN, along with the human immortalized proximal tubule epithelial cell line HK2, human umbilical vein endothelial cells (HUVEC), and HEK293T cells, were sourced from the American Type Culture Collection (ATCC, Manassas, VA).
Techniques: Expressing, Methylation, Western Blot
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